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An automatic tail suspension exam device (Med Associates) was used to measure the period of behavioral immobility. Mice were being suspended because of the tail with tape into a vertical aluminum bar connected to a strain gauge.

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Only homozygous animals had been used in the analyses, although heterozygous mice ended up run in behavioral experiments. Three cohorts of eleven–sixteen mice underwent the 1st battery of behavioral assays for a complete of 42 animals. All mice were initially housed with their littermates of the same sexual intercourse during the Vanderbilt Murine Neurobehavioral Core, with controlled twelve h gentle/darkish cycles, freely readily available foodstuff and water, and managed temperature and humidity. The experimenter was to begin with blinded for the genotype of each animal, and every animal underwent the exact same battery of behavioral assessments to ascertain phenotype.

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